Device

Part:BBa_K935005

Designed by: Twaddle Lab   Group: iGEM12_IvyTech-South_Bend   (2012-10-02)

Two E. coli ArsR generators coupled to pArs-ArsR-RFP

This device contains three coding sequences for the arsenic repressor ArsR under the control of three arsenic promoters pArs with the last promoter also controlling the expression of mRFP1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
    Illegal BglII site found at 918
    Illegal BglII site found at 1581
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2425
    Illegal AgeI site found at 2537
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None